Human Internal Thoracic Artery Smooth M

Application

gene expression, response to injury and proliferation stimuli, blood supply to anterior chest wall and breasts, coronary artery bypass grafting, revascularization

Cell Line Origin

Artery

Components

Basal Medium containing 10% FBS & 10% DMSO

General description

Human Internal Thoracic Smooth Muscle Cells (HITASMC) are derived from the normal internal thoracic arteries (formerly called internal mammary arteries).HITASMC, along with Human Coronary Artery Smooth Muscle Cells, both from Cell Applications, Inc. have been used to investigate the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli (Lange, 2013).Characterization: positive for smooth muscle cell specific alpha-actin expression.

Preparation Note

2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 8 doublings

Subculture Routine

Please refer to the HITASMC Culture Protocol.